Part:BBa_K538302
pBAD + RBS + SheDnaK
This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the DnaK protein from Shewanella sp. AC10 (BBa_K538002) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). Heterologous expression of this protein has rescued E. coli DnaK-null mutants, and facilitated their growth at 15°C. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.
Refer to the main page of the coding region (SheDnaK: BBa_K538002) for more information on how SheDnaK facilitates cold resistance in E. coli.
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 903
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//regulation/positive
function | Protein expression |
output | SheDnaK |
positive_regulators | L-arabinose |