Generator
pBAD:SDnaK

Part:BBa_K538302

Designed by: Bas Stringer   Group: iGEM11_Amsterdam   (2011-09-08)

pBAD + RBS + SheDnaK

pBAD:SDnaK
K538302


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the DnaK protein from Shewanella sp. AC10 (BBa_K538002) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). Heterologous expression of this protein has rescued E. coli DnaK-null mutants, and facilitated their growth at 15°C. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.


Refer to the main page of the coding region (SheDnaK: BBa_K538002) for more information on how SheDnaK facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 903
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//classic/generator/prc
//regulation/positive
Parameters
functionProtein expression
outputSheDnaK
positive_regulatorsL-arabinose